瑞士Cellntec品牌成立于2002年，主研究上皮细胞培养基。Cellntec研究者从上几代人的研究中提炼了见解，并从生态位微环境、控制自我更新、增殖和分化的信号通路以及改善生长因子结合的辅助因子等方面提供了新的方法，从而构建了第五代上皮细胞培养基家族。普瑞麦迪从2013年开始代理瑞士Cellntec品牌到刚刚过去的2018年，已走过6年时光。 感谢国内科研工作者对我们的信赖与支持，也庆幸我们的团队能为国内的科研贡献微薄之力。2019年，该品牌一些产品有相应的调整，此篇为最新资料。详情可登录官网：www.cellntec.com
1.1产品介绍
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上皮细胞 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
代替产品 |
货期 |
CnT-PR
|
CnT-Prime Epithelial Culture Medium |
500mL |
人/鼠 |
皮肤、角膜、牙龈、乳腺、膀胱增殖上皮细胞 |
http://cellntec.com/products/cnt-pr/#datasheet |
CnT-07 CnT-20 CnT-24 |
现货 |
|
CnT-PR-D |
CnT-Prime 2D Diff, Epithelial Culture Medium |
500mL |
人/鼠 |
皮肤、角膜、牙龈、乳腺和膀胱细胞分化上皮细胞 |
http://cellntec.com/products/cnt-pr-d#datasheet |
CnT-02 CnT-30 CnT-32 |
现货 |
|
CnT-PR-3D |
CnT-Prime 3D Barrier Culture Medium |
500mL |
人/鼠 |
人角质形成细胞建立三维表皮模型 |
http://cellntec.com/products/cnt-pr-3d/#datasheet |
CnT-02-3DP |
现货 |
|
CnT-PR-H |
CnT-Prime Keratinocyte Homeostasis Medium |
250mL |
人 |
用于维持原代角质形成细胞在匀浆状态长达1周的培养 |
http://cellntec.com/products/cnt-pr-h/#datasheet |
|
期货 3-4周 |
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CnT-07 |
PCT Epidermal Keratinocyte Medium, Defined |
500mLkit |
人/鼠 |
小鼠和人角质形成细胞的分离和增殖 |
http://cellntec.com/products/cnt-07#datasheet |
CnT-PR |
现货 |
|
CnT-57 |
PCT Epidermal Keratinocyte Medium, Low BPE |
500mLkit |
人/鼠 |
小鼠和人角质形成细胞的分离和增殖。含有低浓度的BPE |
http://cellntec.com/products/cnt-57#datasheet |
CnT-PR |
期货 3-4周 |
|
CnT-09 |
Epidermal Keratinocyte Medium |
500mLkit |
狗 |
用于改善狗的角化细胞生长。 |
http://cellntec.com/products/cnt-09#datasheet |
|
期货 3-4周 |
1.2案例参考
生长在CnT-PR培养基中的原代人角质形成细胞（第5天）
3D表皮模型（用3D Starter Kit建立，第12天）
二、气道细胞系列产品
2.1产品介绍
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气道细胞 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
代替产品 |
货期 |
|
CnT-PR-A |
CnT-Prime Airway, Epithelial Culture Medium |
500mL |
人 |
分离、强增殖和延长初级气道上皮细胞寿命（2D） |
http://cellntec.com/products/cnt-pr-a/#datasheet |
CnT-17 |
期货 3-4周 |
|
CnT-PR-AD |
CnT-Prime Airway Diff, Epithelial Culture Medium |
500mL |
人/鼠 |
促进原发性气道上皮细胞的终末分化（3D） |
http://cellntec.com/products/cnt-pr-ad#datasheet |
CnT-23 |
期货 3-4周 |
|
CnT-17 |
PCT Airway Epithelium Medium, Defined |
500mL kit |
人/鼠 |
小鼠和人气道上皮细胞的分离和增殖（2D） |
http://cellntec.com/products/cnt-17#datasheet |
CnT-PR-A |
期货 3-4周 |
2.2案例参考
生长在CnT-PR-A培养基中的原发性人气道上皮细胞
在CnT-PR-AD培养基中，气道细胞在气液界面分化20天
三、2D＆3D共培养系列产品
3.1产品介绍
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2D＆3D共培养 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-PR-CC |
CnT-Prime Co-Culture, Epithelial/Stromal Co-culture Medium |
250 mL |
人/鼠 |
维持上皮细胞和基质细胞的平衡共培养（2D） |
http://cellntec.com/products/cnt-pr-cc/#datasheet |
期货 3-4周 |
|
CnT-PR-FTAL5 |
CnT-Prime Airlift, Full Thickness Airlift Medium |
500 mL |
人 |
原代人成纤维细胞和角质形成细胞共培养（3D） |
http://cellntec.com/products/cnt-pr-ftal5/#datasheet |
期货 3-4周 |
|
CnT-PR-KM |
CnT-Prime KM, Keratinocyte/Melanocyte Co-Culture Medium |
250 mL |
人 |
角质细胞-黑素细胞共培养培养基 |
http://cellntec.com/products/cnt-pr-km/#datasheet |
期货 3-4周 |
3.2案例参考
角质形成细胞和黑色素细胞共培养（CnT-PR-KM）
四、角化细胞产品
4.1产品介绍
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角化细胞 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-AG2 |
VitroAge Keratinocyte Aging Medium |
500 mL |
人 |
对表皮角质形成细胞进行几次传代的老化处理 |
http://cellntec.com/products/cnt-ag2/#datasheet |
期货 3-4周 |
4.2案例参考
随着年龄的增长，细胞功能的丧失也被认为是细胞增殖的减少
五、MSC系列产品
5.1产品介绍
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MSC |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-PR-MSC |
CnT-Prime MSC Medium |
500 mL |
人 |
骨髓和脂肪来源的骨髓间充质干细胞培养分离和增殖 |
http://cellntec.com/products/cnt-pr-msc/#datasheet |
期货 3-4周 |
|
CnT-PR-MSC-XF |
CnT-Prime MSC Medium, Xeno-Free |
500 mL |
人 |
在完全无氙的环境中高效分离和扩增脂肪来源的MSCs和骨髓来源的MSCs，且无需涂片 |
http://cellntec.com/products/cnt-pr-msc-xf/#datasheet |
期货 3-4周 |
|
CnT-MSCDIFF-AD.S |
Adipogenic Differentiation Supplement |
2 x 1 mL |
人 |
脂肪分化添加剂 |
http://cellntec.com/products/cnt-mscdiff-ad/#datasheet |
期货 3-4周 |
5.2案例图片
adMSC经4代培养后分化为脂肪细胞
六、成纤维细胞系列产品
6.1产品介绍
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成纤维细胞 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-PR-F |
CnT-Prime Fibroblast Proliferation Medium |
500mL kit |
人/鼠 |
成纤维细胞的分离和增殖 |
http://cellntec.com/products/cnt-pr-f#datasheet |
期货 3-4周 |
|
CnT-PR-ECM |
CnT-Prime ECM, Extra Cellular Matrix Medium |
250 mL |
人 |
从一系列组织中分离出的成纤维细胞触发高ECM生成 |
http://cellntec.com/products/cnt-pr-ecm#datasheet |
期货 3-4周 |
|
CnT-PR-FH |
CnT-Prime Fibroblast Homeostasis Medium |
250 mL |
人 |
用于维持原代成纤维细胞在匀浆状态长达1周的培养。 |
http://cellntec.com/products/cnt-pr-fh#datasheet |
期货 3-4周 |
6.2案例参考
全层真皮ECM全层皮肤模型，采用CnTPR-F和CnT-PR-FTAL进行气提培养
生长在CnT-PR-F培养基中的原代人真皮成纤维细胞（第3天）
七、黑色素细胞系列
7.1产品介绍
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黑色素细胞 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-40 |
Melanocyte Medium |
500mL kit |
人 |
黑色素细胞的分离和增殖 |
http://cellntec.com/products/cnt-40/#datasheet |
期货 3-4周 |
|
CnT-PR-MD |
CnT-Prime MD, Melanocyte Differentiation Medium |
250 mL |
人 |
用于分化和增加原代培养人黑素细胞的黑色素表达。细胞必须先在CnT-40培养基中培养到接近汇合处，然后转入CnT-PR-MD培养基诱导分化(5天)。与增殖的CnT-40培养基相比，它能提供2.5倍多的黑色素和增加的树突状细胞。 |
http://cellntec.com/products/cnt-pr-md/#datasheet |
期货 3-4周 |
|
CnT-PR-MH |
CnT-Prime MH, Melanocyte Homeostasis Medium |
250 mL |
人 |
用于维持原代黑色素细胞在匀浆状态长达1周的培养 |
http://cellntec.com/products/cnt-pr-mh/#datasheet |
期货 3-4周 |
7.2案例参考
生长在CnT-40中的原代人黑色素细胞
八、内皮细胞系列
8.1产品介绍
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内皮细胞 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-ENDO |
CnT-Endothelium Medium |
500mL |
人 |
内皮细胞的分离和增殖 |
http://cellntec.com/products/cnt-endo/#datasheet |
期货 3-4周 |
8.2案例参考
生长在CnT-ENDO培养基中的HUVEC内皮细胞
九、iPSC分化系列产品
9.1产品介绍
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iPSC分化 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-30 |
iPS Epithelial Differentiation Medium |
500mL kit |
人 |
用于分化人角膜和表皮上皮细胞的iPSC和ESC |
http://cellntec.com/products/cnt-30/#datasheet |
期货 3-4周 |
|
CnT-PR-EF |
CnT-Prime Epithelial Culture Medium, EGF/FGF-Free |
500mL |
人/鼠 |
人类ESC和iPSC在角膜谱系中的分化 |
http://cellntec.com/products/cnt-pr-ef/#datasheet |
期货 3-4周 |
9.2案例参考
基于CnT-30培养基的人类iPSC协议衍生出的角膜缘SCs。
图片由H提供，Hongisto & H. Skottman(坦培尔大学)
十、辅助试剂
10.1产品介绍
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辅助试剂：抗生素、酶、冻存液、分离液、染色剂 |
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货号 |
英文名 |
规格 |
物种 |
应用 |
说明 |
货期 |
|
CnT-ISO-50 |
CnT-IsoBoost, Isolation Boosting Supplement |
50 uL |
人/鼠 |
可使原代人细胞在分离后第一次传代的分离效率提高一倍以上 |
http://cellntec.com/products/cnt-iso-50/#datasheet |
期货 3-4周 |
|
CnT-ABM10 |
Pen-Strep / Amphotericin B Solution, 250 x, Ready-to-Use Single Aliquots |
10 x 2.0 mL |
人/鼠 |
只建议在隔离期和第1期使用抗生素，不建议在后续的传代中使用 |
http://cellntec.com/products/cnt-abm10#datasheet |
期货 3-4周 |
|
CnT-GAB10 |
Gentamycin / Amphotericin B Solution, 500 x, Ready-to-Use Single Aliquots |
10 x 1.0 mL |
人/鼠 |
只建议在隔离期和第1期使用抗生素，不建议在后续的传代中使用 |
http://cellntec.com/products/cnt-gab10/#datasheet |
期货 3-4周 |
|
CnT-SP |
Spacer Plate, 12-well, resuable |
|
人/鼠 |
|
|
期货 3-4周 |
|
CnT-CRYO-50 |
CRYO Defined, Animal Component Free Freezing Medium |
50 mL |
人/鼠 |
细胞冻存液，用于在冷冻和解冻过程中保护细胞 |
http://cellntec.com/products/cnt-cryo-50#datasheet |
期货 3-4周 |
|
CnT-XP3 |
XP3 Biopsy Maintenance Medium |
250mL |
人/鼠 |
可使组织活检在外植体培养中维持7天以上而不丧失活力或组织结构 |
http://cellntec.com/products/cnt-xp3#datasheet |
期货 3-4周 |
|
CnT-Accutase-100 |
Accutase Cell Detachment Solution |
100 mL |
人/鼠 |
可以作为胰蛋白酶溶液的直接替代品，分离细胞 |
http://cellntec.com/products/cnt-accutase-100#datasheet |
期货 3-4周 |
|
CnT-DNP-10 |
Dispase II, Neutral Protease |
0.5 g |
人/鼠 |
分离上皮细胞和基质细胞 |
http://cellntec.com/products/cnt-dnp-10#datasheet |
期货 3-4周 |
|
CnT-ST-100 |
Stain Kit, incl. Solution I and Solution II |
2 x 50 mL |
人/鼠 |
固定细胞染色 |
http://cellntec.com/products/cnt-st-100#datasheet |
期货 3-4周 |
10.2案例参考
在CnT-PR + IsoBoost补充剂分离后第7天的原发性人角质形成细胞(HPEK)
原代人角质形成细胞解冻后6天恢复
A在传统的20%冷冻介质中冷冻。B冷冻在低温定义的无氙冷冻介质中
在CnT-XP3 (A)中，与竞争对手(B & C)相比，10天后皮肤活检结构保持得更好
CnT-ST-100染色试剂盒评估菌落形成
庆大霉素对细胞培养温度和ph值有一定的耐受性，青霉素和链霉素的降解速度较快
Schafer et al., J. Appl. Microbiol., 1972
如果您科研上有新的想法，又不知道如何解决，可以联系我们，看下面某高校的老师的疑问，我们如何解答的：
1.老师：你好，我现在养小鼠和人的毛囊干细胞，是上皮干细胞的一种，想问你CNT-07适不适合养这种细胞？
大牛: both the CnT-07 and CnT-PR would be the correct media for isolating epidermal progenitor cells from the hair follicle. These media are very similar, but not exactly the same - there are some small difference in growth factors, and the CnT-PR is animal-component-free.
For culture of mouse cells, some labs have found that the CnT-07 has offered slightly better performance. However not all groups have observed this - there are obviously other factors in the protocol that play a role, and some groups like to use specific plate coatings to help retain progenitor cells in an undifferentiated state for longer periods.
Ideally, your customer would be able to compare both media in parallel, for both species. This would allow them to answer the question of whether one of the media works better in their hands. If they were to choose just one medium for both species, then the CnT-07 may be the best choice - however as noted above this is not the case for all research groups.
I hope this helps to clarify further, even though there is not a definitive answer for one or the other medium.（CNT-07适合养毛囊干细胞）
2.老师：我想用分化培养基分化出具有毛发标志角质化细胞，什么培养基可以？
大牛: We have evaluated a range of adhesion markers and other components of the upper layers of the epidermis after 1 week of culture in CnT-PR-D. However we have not checked any hair-related markers. There are many options (as always), and your customer would need to check the expression of those most relevant for them.
Also please keep in mind that some markers are more difficult to find in 2D cultures, and only start to appear when the cells are differentiated in 3D. This could also be a factor for your customer to consider. As always, in many cases only a practical test will give the final answer.
（情况复杂，实际测试才能给出最终答案，我们没有检测出来与毛发相关的标记）
3.老师：我想共培养小鼠和大鼠的真皮和卵泡干细胞，怎么做好？
大牛：if your customer wants to do a co-culture with both epithelial and dermal cells, then the CnT-PR-D wont be ideal, as the dermal cells wont proliferate well.
It will be necessary to use a medium focused on the dermal cells as the base medium - something like CnT-PR-F, and then once the culture is established try a co-culture medium like the CnT-PR-CC. However we have not evaluated the expression of differentiation markers in the CNT-PR-CC, so we dont know exactly what phenotype the cells will demonstrate.
（先用真皮细胞作为基础培养基，然后再用共培养培养基）
以上是2019年最新的产品的产品，这系列的产品近两年来从未涨价，因为厂家原料成本的提升、国内杂费的增加，所以2019年开始我们会微微调价，感谢你们一无既往的支持。小编整理了最近5年内的相关产品的文献，更多文献请登录：
http://cellntec.com/products/resources/tech/publications/?pg=1
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文章名称 |
作者 |
年份 |
组织类型 |
产品货号 |
文章简介链接 |
|
Hongisto H, Ilmarinen T, Vattulainen M, Mikhailova A, Skottman H. |
2018 |
角膜 |
http://cellntec.com/products/resources/tech/publications/xeno-and-feeder-free-differentiation-of-human-pluripotent-stem-cells-to-two-distinct-ocular-epithelial-cell-types-using-simple-modifications-of-one-method-2/ |
||
|
Sorkio A, Koch L, Koivusalo L, Deiwick A, Miettinen S, Chichkov B, Skottman H. |
2018 |
角膜 |
http://cellntec.com/products/resources/tech/publications/human-stem-cell-based-corneal-tissue-mimicking-structures-using-laser-assisted-3d-bioprinting-and-functional-bioinks/ |
||
|
Using Drugs to Probe the Variability of Trans-Epithelial Airway Resistance |
Kendra Tosoni, Diane Cassidy, Barry Kerr, Stephen C. Land, Anil Mehta |
2016 |
气道 |
CnT-17 |
http://cellntec.com/products/resources/tech/publications/using-drugs-to-probe-the-variability-of-trans-epithelial-airway-resistance/ |
|
Wolfgang A. Schulz, Michèle J. Hoffmann and G€unter Niegisch |
2017 |
膀胱 |
CnT-PR, HBLAK |
http://cellntec.com/products/resources/tech/publications/urothelial-carcinoma/ |
|
|
Stefan Aufderklamm,Martin Vaegler,Alexandra Kelp,Sabine Maurer,Leon Gustafsson,Jens Mundhenk,Silke Busch,Lisa Daum,Arnulf Stenzl,Bastian Amend,Karl‑Dietrich Sievert |
2017 |
膀胱 |
CnT-07 |
http://cellntec.com/products/resources/tech/publications/collagen-cell-carriers-seeded-with-human-urothelial-cells-for-urethral-reconstructive-surgery-first-results-in-a-xenograft-minipig-model/ |
|
|
The mitochondrial respiratory chain is required for organismal adaptation to hypoxia |
Robert B. Hamanaka, Samuel E. Weinberg, Colleen R. Reczek, Navdeep S. Chandel |
2016 |
表皮 |
CnT-07 |
http://cellntec.com/products/resources/tech/publications/the-mitochondrial-respiratory-chain-is-required-for-organismal-adaptation-to-hypoxia/ |
|
A lung-on-chip array with an integrated bio-inspired respiration mechanism |
Andreas O. Stucki, Janick D. Stucki, Sean R. R. Hall, Marcel Felder, Yves Mermoud, Ralph A. Schmid, Thomas Geiser, Olivier T. Guenat |
2014 |
气道 |
CnT-PR-A |
http://cellntec.com/products/resources/tech/publications/a-lung-on-chip-array-with-an-integrated-bio-inspired-respiration-mechanism/ |
|
A TALEN-mediated, p63 knock-in application in human iPS cells |
Kobayashi, Y, Hayashi, R, Quantock, Andrew and Kohji Nishida, K |
2017 |
角膜 |
CnT-PR |
http://cellntec.com/products/resources/tech/publications/a-talen-mediated-p63-knock-in-application-in-human-ips-cells/ |
|
Harry Horsley, Dhanuson Dharmasena, James Malone-Lee & Jennifer L. Rohn |
2017 |
膀胱 |
CnT-PR, CnT-PR-3D, HBLAK |
http://cellntec.com/products/resources/tech/publications/a-urine-dependent-human-urothelial-organoid-offers-a-potential-alternative-to-rodent-models-of-infection/ |
|
|
Marta N. Shahbazi, Daniel Peña-Jimenez, Francesca Antonucci, Matthias Drosten and Mirna Perez-Moreno |
Marta N. Shahbazi, Daniel Peña-Jimenez, Francesca Antonucci, Matthias Drosten and Mirna Perez-Moreno |
2017 |
表皮 |
CnT-57, CnT-07 |
http://cellntec.com/products/resources/tech/publications/clasp2-ensures-mitotic-fidelity-and-prevents-differentiation-of-epidermal-keratinocytes/ |
|
Rikke Christensen, David M. Owens, Annette C. Füchtbauer, Anders Gunnarsson, Mette Ramsing, Ernst-Martin Füchtbauer, Uffe Birk Jensen |
2017 |
表皮 |
CnT-57 |
http://cellntec.com/products/resources/tech/publications/constitutive-transgene-expression-of-stem-cell-antigen-1-in-the-hair-follicle-alters-the-sensitivity-to-tumor-formation-and-progression/ |
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Cystic Fibrosis Transmembrane Conductance Regulator dysfunction in VIP knockout mice |
Nicole G Alcolado, Dustin J Conrad, Diogo Poroca, Mansong Li, Walaa Alshafie, Frederic G Chappe, Ryan M Pelis, Younes Anini, Zhaolin Xu, Sayyed Hamidi, Sami I Said & Valerie M Chappe |
2014 |
气道 |
CnT-17 |
http://cellntec.com/products/resources/tech/publications/cystic-fibrosis-transmembrane-conductance-regulator-dysfunction-in-vip-knockout-mice/ |
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Engineering human cell spheroids to model embryonic tissue fusion in vitro |
David G. Belair, Cynthia J. Wolf, Carmen Wood, Hongzu Ren, Rachel Grindstaff, William Padgett, Adam Swank, Denise MacMillan, Anna Fisher, Witold Winnik, Barbara D. Abbott |
2017 |
表皮 |
Primary HPEKp CnT-PR Accutase CnT-CRYO-50 CnT-PR-CC |
http://cellntec.com/products/resources/tech/publications/engineering-human-cell-spheroids-to-model-embryonic-tissue-fusion-in-vitro/ |
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Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes |
Lilian Julia Löwenau, Christian Zoschke, Robert Brodwolf, Pierre Volz, Christian Hausmann, Suvara Wattanapitayakul, Alexander Boreham, Ulrike Alexiev, Monika Schäfer-Kortin |
2017 |
表皮 |
CnT-PR-3D |
http://cellntec.com/products/resources/tech/publications/increased-permeability-of-reconstructed-human-epidermis-from-uvb-irradiated-keratinocytes/ |
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Yoriko Iwamoto, Keizo Nishikawa, Ryusuke Imaia,, Masayuki Furuya, Maki Uenaka, Yumi Ohta, Tetsuo Morihana, Saori Itoi-Ochi, Josef M. Penninger, Ichiro Katayama, Hidenori Inohara and Masaru Ishii |
2016 |
表皮 |
CnT-PR, CnT-PR-3D |
http://cellntec.com/products/resources/tech/publications/intercellular-communication-between-keratinocytes-and-fibroblasts-induces-1-local-osteoclast-differentiation-a-mechanism-underlying-2-cholesteatoma-induced-bone-destruction/ |
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PNPLA1 is a transacylase essential for the generation of the skin barrier lipid o-O-acylceramide |
Ohno Y, Kamiyama N, Nakamichi S, Kihara A |
2017 |
表皮 |
HPEKp,CnT-PR, CnT-PR-3D |
http://cellntec.com/products/resources/tech/publications/pnpla1-is-a-transacylase-essential-for-the-generation-of-the-skin-barrier-lipid-o-o-acylceramide/ |
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Spatial Distributionof StemCell-Like keratinocytes in Dissected Compound Hair Follicles of the Dog |
Dominique J. Wiener, Marcus G. Doherr, Eliane J. Müller, Monika M. Welle |
2016 |
表皮 |
CnT-09 |
http://cellntec.com/products/resources/tech/publications/spatial-distributionof-stemcell-like-keratinocytes-in-dissected-compound-hair-follicles-of-the-dog/ |
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更多文献请访问：http://cellntec.com/products/resources/tech/publications/?pg=1 |
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