Palm PCR：Fully portable,ultra-fast device with extreme efficiency and convenience

Technology Key Palm PCR is a battery-powered, palm-size portable PCR machine. This unprecedented device features a fully portable, stand-alone thermocycler that delivers PCR amplication with high speed and sensitivity in a small handheld format. In marked contrast to the conventional thermal cycling, there is no temperature ramping in the Palm PCR device, thus not requiring the huge, hundreds of watts of electric power and cumbersome programming of many parameters typical in the conventional PCR machines.

Palm PCR device features three stages of specially structured heat blocks that are maintained at temperatures suitable for each of the three PCR steps, and the sample is circulated across the high and low&temperature zones inside a sample tube. Ahram’s proprietary Palm PCR technology enables accurate and robust control of the sample circulation for performing ultra-fast PCR amplication.

Palm PCR Control

A protocol to perform a PCR reaction in the Palm PCR device can be programmed in a simple, convenient manner. It requires setting up only four control parameters: PCR speed, Annealing temperature, PCR time, and Preheat. Other control parameters such as melting and extension temperatures are automatically controlled by the operating software. Annealing temperature can be selected in the range from 52.0°C to 60.0°C.

PCR Speed and Dynamic Range

In the Palm PCR device, speed of the thermal cycling is controlled by setting the PCR speed level. Seven PCR speed levels available are categorized into the follow -ing three modes:

Standard Fast mode: F1, F2 and F3

Standard Slow mode: S1 and S2

Turbo Fast mode: T1 and T2

Selecting a PCR speed level makes the Palm PCR device perform PCR amplication with a pre&dened speed as listed in the Palm PCR Selection Chart. Dynamic range of the amplication depends on the PCR speed level. It is broader at a lower speed and narrower at a higher speed.

Table1：Palm PCR Mode and Speed

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