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首页 | 试剂 | 细胞培养

转染试剂


Viromers®优势:
a.
避免包涵体吞噬:最大化转染效率并降低背景;
b.
零电荷:无需去除血清和抗生素,对细胞温和;
c.
稳定:实验结果具有重复性;
d.
反向转染:适用于高通量筛选;
e.
无脂质:适用于脂肪代谢活跃的细胞,如,脂肪细胞或肝细胞。

产品特点:
高转染效率,高细胞存活率。


Viromers® RED /YELLOW jetPRIME, FuGENE, Lipofectamine Mirus X2 的转染效率比较
使用Viromer® RED /YELLOW及其它转染试剂,转染pCMV-Luc (50ng DNA/96-well)到标准细胞系中,检测荧光素酶表达及细胞存活率,发现Viromers® RED /YELLOW转染HelaCHOHEX获得更高的转染效率。

应用实例1
SH-SY5Y neuroblastoma

使用Viromer®RED, FuGene ®HD or TransIT-X2™ 转染eGFP 质粒 (100ng DNA/96-well)SH-Sy 5yneuroblastoma24h后的FACS及镜检结果

应用实例2
RAW 264.7 mouse macrophages

使用Viromer®RED, FuGene ®HD or TransIT-X2™ 转染eGFP 质粒 (100ng DNA/96-well)RAW264.7 mouse macrophages24h后的FACS及镜检结果

订购信息:

货号

品名

规格

价格

备注

VR-01LB-00

Viromer® RED

80 T in 24 well

1000

已验证细胞系
参照表3

VR-01LB-01

0.18 ml600T

7140

VR-01LB-03

3 x 0.18 ml1800T

18360

VY-01LB-00

Viromer® YELLOW

80 T in 24 well

1000

VY-01LB-01

0.18 ml600T

7140

VY-01LB-03

3 x 0.18 ml1800T

18360

3. Viromer® RED & YELLOW cell 验证细胞系

Category

Cell type

Viromer®

Cell lines

Standards: HeLa, Neuro2A, CHO, HepG2, HEK-293 …

RED & YELLOW


HT-29 colorectal carcinoma

RED

A549 lung adenocarcinoma

RED

H226 lung squamous cell carcinoma

RED

BEAS-2B bronchial epithelial

RED

HACAT keratinocytes

RED

Huh-7 hepatocellular carcinoma

RED

SH-SY5Y neuroblastoma

RED

INS-1 insulin secreting beta cell derived line

YELLOW

Suspension cells

THP-1 monocytes

RED

Primary & Stem cells

Primary mouse hepatocytes

RED


Primary keratinocytes

RED

Primary human macrophages

RED

Primary cardiomyocytes

YELLOW

Primary fibroblasts

YELLOW

Primary myoblasts

RED & YELLOW

温馨提示:不可用于临床治疗。

Viromers® Green&Blue
Viromers® Green/Blue
均适用于siRNAmiRNA转染,分别适用于不同的细胞系(贴壁细胞或悬浮细胞),均表现出高转染效率。

使用Viromer®BLUE /GREEN , Lipofectamine转染siRNA 到原代小鼠成纤维细胞中,检测目标基因和GAPDH mRNA的表达

使用Viromer®BLUE /GREEN , Lipofectamine转染siRNA cancer stem cells / mammospheres中,检测目标基因和GAPDH mRNA的表达

反向转染表现优越,适用于高通量筛选

Viromer®BLUE转染原代巨噬细胞,在384孔板中,使用Viromer®BLUE转染siRNA targeting EEA1到原代巨噬细胞(从buffy coat PBMC 现分离)中,检测endosomes 水平(siRNA targeting EEA1会导致endosomes 水平降低)

订购详情:

货号

品名

规格

价格

备注

VB-01LB-00

Viromer® BLUE

80 T in 24 well

1000

已验证细胞系
参照表6

VB-01LB-01

0.3 ml500T

5580

VB-01LB-03

3 x 0.3 ml1500T

14160

VG-01LB-00

Viromer® GREEN

80 T in 24 well

1000

VG-01LB-01

0.3 ml500T

5580

VG-01LB-03

3 x 0.3 ml1500T

14160

. Viromer® Blue and Green cell preferences

Category

Cell type

Viromer®

Cell lines

Standards: HeLa, Neuro2A, CHO, A549, HepG2, HEK-293

BLUE &GREEN


MCF-7 breast adenocarcinoma

BLUE

RAW264.7 & NIH3T3

BLUE

U87, NCH82 & NCH149 glioblastoma

BLUE &GREEN

MDA-MB468 &-MB231 breast adenocarcinoma

BLUE &GREEN

CT26, HT-29, HCT116 colon carcinoma

BLUE &GREEN

P4E6 prostate carcinoma

BLUE

Hs746T gastric carcinoma

BLUE &GREEN

MPC mouse pheochromocytoma cells

BLUE &GREEN

SH-SY5Y neuroblastoma

GREEN

MRC-5 lung fibroblasts

GREEN

TEU-2 immortilizedurothelial cells

GREEN

C2C12 myoblasts

BLUE

U2OS osteosarcoma

BLUE

Suspension cells

THP-1 monocytes

BLUE &GREEN


CHO-K1 suspension

GREEN

Kasumi-4 & MV4-11 myelomonocytic leukemia cells

BLUE

Primary & Stem cells

Pre-adipocytes (3T3-L1, C3H10T1/2) & adipocytes

BLUE


Schwann cells from mouse brain

BLUE

Primary mouse hepatocytes

BLUE

Primary human macrophages & PBMCs

BLUE

Primary fibroblasts

BLUE &GREEN

Primary human skeletal myoblasts

BLUE

Primary human bladder smooth muscles

BLUE

HUVECs

BLUE

Cancer stem cells / 3D-mammospheres

BLUE

Dental follicle stem cells

GREEN

Special applications

3D-mammosphere / Co-culture transfection

BLUE


Disaggregated primary human xenograft tumor cells

BLUE

温馨提示:不可用于临床治疗。